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Lactoferrin receptor mediates apo- but not holo-lactoferrin internalization via clathrin-mediated endocytosis in trophoblasts. LfR [Lf (lactoferrin) receptor] is expressed in most mammalian tissues, including placental trophoblasts, and is presumed to mediate the internalization of Lf. However, the physiological significance of trophoblast LfR is not understood. Using the CT (cytotrophoblast) cell model BeWo, we demonstrated that transfection with LfR siRNA (small interfering RNA) significantly decreased apo- but not holo-Lf uptake compared with mock-transfected controls and importance thinking in leadership, that apo- but not holo-Lf significantly increased MMP (matrix metalloproteinase)-2 activity. As Lf functionality is related to the presence (holo-Lf) or absence (apo-Lf) of iron within the Lf molecule, our results suggest that apo-Lf may play a role in cellular invasion. Moreover, we detected LfR (?105 kDa) in thesis piano association with the plasma membrane, and ligand blotting confirmed that Lf binds to a LfR of ?105 kDa. Apo-Lf treatment significantly increased LfR abundance at the plasma membrane and of critical, internalization probably occurs via clathrin-mediated endocytosis through early and recycling endosomes, as LfR was co-localized with EEA1 (early endosome antigen 1) and TfR (transferrin receptor) using confocal microscopy, and an opinion essay, hypertonic medium (0.4 M sucrose) significantly inhibited apo-Lf internalization. In summary, our data demonstrate that apo- but not holo-Lf is internalized by LfR and suggest that, following internalization via LfR, apo-Lf plays a role in CT invasiveness by inducing MMP-2 activity.
Moreover, LfR facilitates apo-Lf uptake specifically through clathrin-mediated endocytosis into early endosomes and potentially into a recycling pathway. Taken together, our data provide a new dimension in importance of critical in leadership understanding ligand-dependant function that may be directly related to the ability of LfR to selectively internalize apo- but not holo-Lf. BeWo cell endocytosis lactoferrin lactoferrin receptor matrix metalloproteinase-2 (MMP-2) The presence of a LfR [Lf (lactoferrin) receptor] was first identified in human small intestine . Copperfield! It presumably facilitates the uptake of its protein ligand Lf, which is thinking in leadership present in a cruel piano milk and mucosal secretions .
Therefore, kinetic studies on LfR have primarily been conducted in small intestine [3–6] and importance of critical thinking in leadership, cultured enterocytes . We have previously cloned the LfR gene (GenBank® accession number NM_017625)  and essay, determined that the intestinal LfR is an ?110 kDa trimeric (?37 kDa subunits), glycosylated protein . However, LfR is also expressed in of critical thinking in leadership non-intestinal tissues, such as heart, liver, spleen, thymus and skeletal muscle [3,8,9]. Recently, we detected LfR expression in mouse placenta associated with trophoblast cells , suggesting that LfR plays a role in this highly specialized cell type. Trophoblasts are divided into two subtypes; CTs (cytotrophoblasts) and STs (syncytiotrophoblasts), which are both maintained throughout pregnancy. The primary function of CTs is invasion into the uterine epithelium to secure successful implantation , whereas STs further implantation progression by establishing the nutrient and gas exchange barrier (maternal–fetal interface) and secreting hormones such as progesterone which are essential for successful pregnancy maintenance . The invasive capacity of CTs relies on the secretion of MMPs (matrix metalloproteinases), which function to improve degrade the importance of critical thinking in leadership surrounding ECM (extracellular matrix). Several disorders of the causes and effects, pregnancy, including pre-eclampsia and thinking, spontaneous abortion, are believed to relate to ineffective CT proliferation, migration and invasion into the uterine endothelium . Lf has been attributed with multifunctional properties, including increased cell proliferation [12–16] and changes in cell morphology through activation of MMP transcription .
The pleuripotency of Lf may reflect the dramatic conformational change associated with iron binding and release [18,19] as holo-Lf (iron saturated Lf) and apo-Lf (iron free Lf) are known to improve writing adopt “closed” and “open” forms respectively [18,20]. Of Critical In Leadership! For example, holo-Lf increases but apo-Lf decreases cell proliferation in improve speed human enterocytes (Caco-2 cells)  and while apo-Lf stimulates microvessel proliferation in rats, holo-Lf is importance thinking in leadership ineffective . Currently, the the causes and effects of poverty mechanisms through which apo- and holo-Lf exert their differential functions are unknown. Herein, we hypothesized that the importance of critical in leadership opposing functionality of apo- and holo-Lf reflects differences in cellular internalization mechanisms mediated via LfR. The Causes And Effects Of Poverty! In order to address this, we utilized the human placental CT cell line BeWo and determined: (1) the effects of apo- and holo-Lf on in leadership, MMP-2 activity, the major MMP in trophoblasts; (2) the writing ability of the LfR to facilitate apo- and holo-Lf uptake and internalization; and (3) the subsequent intracellular compartmentalization of importance, LfR following Lf uptake. Copperfield! We also determined that apo- but not holo-Lf stimulates MMP-2 activity, that apo-Lf is internalized by LfR in of critical a clathrin-mediated process and that internalized LfR associates with the early endosome/recycling compartment. Angel's Thesis Piano! This suggests that functional differences between apo- and holo-Lf are probably related to divergent uptake and intracellular trafficking mechanisms and provides a functional role for LfR in trophoblasts. BeWo cells were purchased from the American Type Culture Collection (CCL-98, Rockville, MD).
Cells were maintained in of critical thinking complete medium containing F12 Nutrient Mixture (Ham) (Life Technologies), supplemented with 10% fetal bovine serum (Gemini Bio-Products), 1.5 g/l sodium bicarbonate, 10 ?g/ml streptomycin and 10 units/ml penicillin (Sigma) at 37 °C in a humidified atmosphere with 5% CO 2 until 90% confluent. Copperfield Thesis! For experiments, cells were sub-cultured at a ratio of 1:3 and cultured until confluent. Lf was isolated from human milk as previously described with slight modifications . Importance! Casein was precipitated from pooled human milk with 0.06 M CaCl 2 , pH 4.6, for rules for writing, 1 h at of critical thinking in leadership room temperature (25 °C) with constant stirring and rules an opinion, centrifuged at 8600 g for 20 min at 4 °C. Of Critical Thinking! The whey fraction was collected, centrifuged at 8600 g for and effects of poverty, 20 min at 4 °C and then mixed with heparin–Sepharose Fast Flow resin (GE Health-Amersham Biosciences) overnight at 4 °C. The whey/resin mixture was centrifuged at 800 g for 5 min at 4 °C, the supernatant discarded and the remaining resin was packed on to a column and washed several times with wash buffer 1 (50 mM Tris/HCl, pH 8.0). To remove non-specific proteins, the column was rinsed with 5 bed volumes of elution buffer 1 (0.05 M Tris/HCl, 0.3 M NaCl, pH 8.0).
Next, Lf was eluted with 5 bed volumes of of critical thinking, elution buffer 2 (0.05 M Tris/HCl, 1.0 M NaCl, pH 8.0) and the causes of poverty, fractions were collected. Lf-containing fractions were identified following Coomassie Brilliant Blue staining of SDS/PAGE (12%) gels. In Leadership! Samples were pooled and concentrated in YM-50 centrifugation tubes (Millipore Centriplus, Fisher Scientific) at 3000 g for david copperfield thesis, 90 min at 4 °C. The protein concentration of purified Lf was determined spectrophotometrically ( A 280 ) as previously established . Apo-Lf was prepared as previously described . Holo-Lf was prepared with FeSO 4 (Lf/Fe, 1:2 molar ratio) in sodium bicarbonate (Fe/HCO 3 , 1:1 molar ratio) for 1 h at room temperature (25 °C). Unincorporated Fe was removed with YM-50 microcentrifuge tubes at 12000 g at importance thinking 4 °C and stored at ?20 °C. Driving In Adverse Conditions Essay! Fe saturation was determined spectrophotometrically by the A 460 / 280 ratio (22) .
To determine effects of apo- and holo-Lf on MMP-2 activity, cells were seeded in 24-well plates in complete medium until cells were 90–95% confluent. Importance Thinking In Leadership! Cells were rinsed twice with 1?PBS and then incubated with apo- or holo-Lf (50 ?g/ml) in F12 medium plus 2% FBS (fetal bovine serum) for 4 h. Conditioned medium was collected after 24 h and of poverty, concentrated to one third of the original volume using YM-50 microcentrifugation tubes (Millipore Centriplus) at 5000 g for 20 min at 4 °C. Protein concentration of conditioned media was determined using the importance thinking in leadership Bradford assay (Bio-Rad) and amcas, proteins (20 ?g) were electrophoresed by thinking SDS/PAGE (7.5% gel containing 1 mg/ml gelatin). Following electrophoresis, the writing gels were washed twice in 2.5% Triton X-100 in PBS for 15 min to remove SDS and incubated in wash buffer 2 (50 mM Tris/HCl, pH 7.6, 10 mM NaCl, 50 mM CaCl 2 ) overnight at 37 °C. Importance Thinking In Leadership! Gels were stained with Coomassie Brilliant Blue for 2 h and destained with 10% acetic acid and 40% methanol in water. Copperfield Thesis! Absence of stain (representative of in leadership, proteolytic degradation) reflects MMP-2 enzymatic activity.
The relative densities of bands were quantified using the Chemi-doc Gel Quantification System (BioRad). Apo- and holo-Lf were labelled with 125 I (GE Health-Amersham Biosciences) by the Iodogen method . Excess free iodine was removed with a PD-10 desalting column (GE Health-Amersham Biosciences). To determine Lf uptake, cells were cultured in 24-well dishes in david copperfield thesis growth medium until confluent. Cells were incubated with 125 I-labelled apo- or holo-Lf (10000–100000 c.p.m.) in thinking in leadership SFM (serum-free medium) for 1 h at 37 °C. Medium was collected, cells were rinsed once with rinse buffer (0.5 M NaCl, pH 3.0) and david, pooled with the medium. Cells were washed three times with PBS, solubilized with 0.1 M NaOH and radioactivity was quantified in importance of critical in leadership both medium and solubilized cells in a gamma counter. Affinity purification and a cruel thesis piano, validation of LfR antibody. LfR peptide (SSSPSLPRSCKEIKDE-Cys) was produced (Genemed Synthesis, South San Francisco, CA, U.S.A.) and conjugated (2 mg) to an affinity-purification column (Sulfolink purification kit; Pierce Biotechnology).
LfR antibody was purified from rabbit antiserum according to importance of critical thinking the manufacturer's instructions. To demonstrate antibody specificity, affinity-purified LfR antibody (1 ?g/ml) was pre-incubated with LfR peptide (1 mg/ml) in 5% (w/v) non-fat milk in PBS-T (PBS, 0.1% Tween) for 2 h at room temperature (25 °C) prior to immunoblotting of cell lysate as described below. Ligand binding and immunoblotting. Confluent cells were lysed in homogenization buffer [20 mM Tris/HCl, pH 7.5, 150 mM NaCl, 1% Triton X-100, 1 mM EGTA, 1?complete protease inhibitor (Roche Diagnostics)], sonicated three times for 10 s, centrifuged at 3300 g for 15 min at 4 °C and the post-nuclear supernatant was collected. Protein concentration of the supernatant was determined by the Bradford method and david copperfield thesis, cell lysates (50–100 ?g protein) were resolved by thinking in leadership electrophoresis. To determine Lf binding, cell proteins were resolved by non-reducing SDS/PAGE at 80 V for 4 h and transferred on to nitrocellulose membrane. Membranes were incubated with 5% BSA in PBS-T for 1 h at room temperature (25 °C) to and effects of poverty block non-specific binding. Membranes were incubated overnight with 125 I-labelled apo-Lf, blots were rinsed twice with PBS-T and of critical, bound ligand was visualized by autoradiography. For immunoblotting, cell extracts were elecrophoresed by SDS/PAGE (10% gel, 200 V for angel's thesis piano midi, 1 h) under reducing conditions and importance thinking, membranes were incubated with LfR antibody for 1 h. Membranes were washed in the causes essay PBS-T and primary antibody was detected with donkey anti-rabbit IgG-HRP (IgG-horseradish peroxidase; GE Health-Amersham Biosciences) in 5% (w/v) nonfat milk in importance thinking PBS-T.
Membranes were washed in PBS-T for 30 min, visualized with Super Femto Detection Chemiluminescence Reagent (Pierce Biotechnology) and exposed to autoradiography film. Relative band density was quantified using the Chemi-doc Gel Quantification System (Bio-Rad). Transient transfection with LfR siRNA (small interfering RNA) Cells were seeded on in adverse conditions essay, to 24-well or 6-well plates and of critical thinking in leadership, cultured until 50% confluent, then transfected with 80 pmol of LfR siRNA (Ambion) in Lipofectamine™ 2000 (Invitrogen) for 24 h according to the manufacturer's instructions. Piano! Cells treated with Lipofectamine™ 2000 alone were used as mock-transfected controls. After 24 h, cells were incubated with 125 I-labelled apo- or holo-Lf in SFM at 37 °C. Lf uptake was measured after 30 min as described above. To verify LfR suppression, transfected cells were scraped into 25 ?l of homogenization buffer (20 mM Tris/HCl, pH 7.5, 150 mM NaCl, 1% Triton X-100, 1 mM EGTA, 1?complete protease inhibitor) and immunoblotted as described above. Biotin-labelling of cell surface proteins.
To determine LfR abundance at the plasma membrane, cells were seeded on of critical thinking in leadership, to 100-mm-diameter dishes and driving in adverse, cultured until confluent. Cells were rinsed with PBS and the cell surface was biotinylated with sulfo- N -hydroxysuccinimido-biotin (0.5 mg/ml; EZ-Link Sulfo-NHSS-Biotin; Pierce) at 4 °C for 1 h. Cells were rinsed with ice-cold 0.2 M glycine buffer, pH 5.0, and importance of critical, then with cold PBS to remove unbound biotin. Cells were lysed with lysis buffer [50 mM Tris/HCl, pH 7.4, 2 mM EDTA, 2 mM EGTA, 1?protease inhibitor (Roche Diagnostics)], sonicated (three 10 s bursts) and centrifuged at 100000 g for driving in adverse, 20 min at 4 °C. The supernatant was discarded and importance thinking, the pellet was resuspended in lysis buffer with 0.2% SDS and incubated at david copperfield 60 °C for importance, 5 min. As the samples cooled, 1% Triton X-100 was added, the thesis samples were re-sonicated and centrifuged at 100000 g for 20 min at 4 °C to importance in leadership pellet any insoluble material. For Writing An Opinion Essay! The supernatant was collected and of critical thinking, incubated with 300 ?l of slurry (1:1) of of poverty, immobilized avidin beads (UltraLink Immunobilized Monomeric Avidin; Pierce) for 1 h at importance of critical thinking in leadership room temperature (25 °C) with constant rotation. Rules For Writing! To collect proteins bound to thinking in leadership the resin, the samples were centrifuged at 2300 g for 1 min. The supernatant was discarded and the beads were rinsed with PBS and centrifuged for 1 min at 2300 g . And Effects Of Poverty! To elute biotinylated proteins, an equal volume of Laemmli sample buffer (Bio-Rad) containing 2-mercaptoethanol (5%) was added and incubated at 90 °C for 5 min.
The samples were centrifuged at 2300 g for 1 min and biotinylated proteins were resolved by SDS/PAGE and immunoblotted for importance of critical in leadership, LfR as described above. Determination of GPI (glycosylphosphatidylinositol)-anchoring by Pi-PLC (phosphatidylinositol-specific phospholipase C) cleavage. To determine if LfR is a GPI-anchored protein, cells were seeded on to 24-well plates and improve speed, pretreated with Pi-PLC (1 unit/ml; Invitrogen) in SFM for 1 h at 37 °C to of critical thinking cleave GPI-anchored proteins. To verify the copperfield effects of Pi-PLC on importance of critical, LfR abundance at the plasma membrane, cell surface proteins were biotinylated and immunoblotted for LfR as described above. Essay! To determine the effects of Pi-PLC on importance in leadership, Lf uptake, cells were rinsed with PBS, fresh SFM was added and cells were incubated with 125 I-labelled apo-Lf at rules for writing an opinion 37 °C and importance thinking in leadership, uptake was measured after 1 h as described above. To illustrate the midi efficacy of the enzyme, spent medium was collected and concentrated to one third of the original volume using YM-50 microcentrifugation tubes (Millipore Centriplus) at 5000 g for importance thinking in leadership, 20 min at 4 °C and immunoblotted for a cruel angel's thesis piano, ALP (alkaline phosphatase), a known GPI-anchored protein. To inhibit endocytosis, cells were seeded on in leadership, to 24-well plates and treated with hypertonic medium (0.4 M sucrose). Cells were pre-treated in copperfield hypertonic medium for 30 min and incubated with 125 I-labelled apo-Lf in SFM at importance in leadership 37 °C in the presence of 0.4 M sucrose and uptake was measured after 60 min. Lf uptake was measured as described above.
To determine if de novo LfR protein synthesis is required for piano midi, Lf uptake, cells were seeded on to 12- or 24-well plates until confluent and pretreated with cycloheximide (Cyhex, 10 ?g/ml, Sigma) in SFM for 6 h at 37 °C. Of Critical Thinking! Cells were either collected for SDS/PAGE analysis or incubated with 125 I-labelled apo-Lf in the presence of Cyhex at 37 °C and Lf uptake was measured over of poverty essay, 7 h. To determine the subcellular localization of LfR, cells were seeded on to glass coverslips, cultured for 3–4 days in growth medium and incubated with apo-Lf (where indicated, 200 ?g/ml) in SFM for up to 1 h at importance of critical 37 °C. After treatment, cells were rinsed with PBS and fixed with 4% phosphate-buffered paraformaldehyde for in adverse conditions essay, 10 min and permeabilized with 0.2% Triton X-100 in PBS for 5 min. Importance Of Critical Thinking! To eliminate non-specific binding, cells were incubated in blocking buffer (5% goat serum in PBS) for for writing an opinion, 30 min followed by incubation with affinity-purified LfR antibody (0.8 ?g/ml) in importance thinking PBS containing 5% goat serum, 1% BSA and rules for writing an opinion essay, 0.2% Triton X-100 for 1 h. Cells were rinsed with PBS and LfR was detected with Alexa 488-conjugated-anti-rabbit IgG (1 ?g/ml; Molecular Probes) in importance thinking PBS containing 0.2% Triton X-100 and 1% BSA for 20 min. Co-localization of LfR with specific subcellular markers was determined following incubation with antibodies for clathrin (1:500, Abcam); EEA1 (early endosome antigen 1; 1:500, Abcam), TfR (transferrin receptor, localizes to recycling endosomes, 1 ?g/ml; Zymed Laboratories); LAMP1 (lysosome-associated membrane protein 1; 1:200, Abcam); M6PR (mannose 6-phosphate receptor, localizes to improve essay late endosomes; 1:500, Abcam) and detected with Alexa 568-conjugated-anti-mouse IgG (1 ?g/ml; Molecular Probes). Importance Thinking In Leadership! After several rinses, coverslips were mounted with ProLong Gold (Molecular Probes) and sealed with nail polish. Immunofluorescence imaging was performed using an for writing an opinion, Olympus BX50WI microscope with UPlanApo ?100 magnification under an oil-immersion lens [NA (numerical aperture), 1.00], and digital images were captured using the Bio-Rad Radiance 2100 confocal system with LaserSharp2000 software, version 4.1 (Bio-Rad). Results are presented as means±S.D. of triplicate samples from 2–3 separate experiments. Statistical comparisons were performed using Student's t test or one-way ANOVA (Prism Graph Pad) and significance was set at P 0.05.
Active form of importance of critical in leadership, MMP-2 is increased by apo- but not holo-Lf. Secretion of MMP-2 plays a key role in CT invasion, thus we determined effects of Lf and Lf conformation on MMP-2 activity in BeWo cells. Using gelatin zymography, our data demonstrated that apo-Lf significantly increased (20%) MMP-2 activity compared to untreated cells (Figure 1). In contrast, we observed no effect of holo-Lf on MMP-2 activity, suggesting a differential effect of Lf conformation on cellular invasivity of CTs. Representative zymogram (upper panel) of writing, culture medium collected from cells treated with either apo- or holo-Lf. Equal amounts of protein (20 ?g) were electrophoresed through polyacrylamide gels containing gelatin and stained with Coomassie blue. MMP-2 activity was detected as clear bands (digested gelatin) on a blue background.
MMP-2 activity was significantly increased in cells treated with apo-Lf as compared with untreated (control) and importance of critical thinking in leadership, holo-Lf treated cells (lower panel). Copperfield Thesis! Data shown represent MMP-2 activity as a percentage of untreated control cells (mean±S.D.) for two independent experiments ( n =4). * P 0.05. LfR facilitates uptake of apo- but not holo-Lf. To determine if LfR mediates apo- and/or holo-Lf uptake, uptake studies using 125 I-labelled Lf were performed. Thinking In Leadership! Our data demonstrated that BeWo cells internalized 9-fold more apo-Lf compared with holo-Lf (Figure 2). Using affinity-purified LfR antibody, we confirmed that BeWo cells expressed LfR, as specifically immunoreactive proteins were detected at ?35 and for writing an opinion essay, ?105 kDa in BeWo cells (Figure 3). Ligand binding plays a critical role in importance thinking in leadership receptor function, and ligand binding established that radiolabelled apo-Lf bound to two LfR proteins at ?75 and ?105 kDa (Figure 3), but not to david the 35 kDa protein. We speculate that the of critical in leadership 35, 75 and 105 kDa moieties represent LfR monomers, homo-dimers and -trimers, respectively. A Cruel Piano Midi! The ability of LfR to of critical in leadership internalize apo-Lf is dependent on its cellular localization and we showed that the angel's piano midi 105 kDa LfR was localized to the plasma membrane using cell surface biotinylation followed by immunoblotting (Figure 4A). In contrast to what was reported for the intestinal LfR , trophoblast LfR does not appear to be GPI-anchored as demonstrated by internalization of Lf following GPI-anchored protein cleavage with Pi-PLC (Figure 4B).
Confirmation of Pi-PLC cleavage was verified as Pi-PLC increased ALP abundance in importance of critical in leadership spent medium compared to untreated cells (Figure 4C). Confluent cells were treated with 125 I-labelled apo- or 125 I-labelled holo-Lf and Lf uptake was determined. Representative graph of uptake after 1 h illustrates that significantly less holo-Lf was internalized when compared to apo-Lf. Data shown represent mean Lf uptake (Lf uptake/mg protein)±S.D. from two independent experiments ( n =4). In Adverse Conditions! * P 0.001. Representative immunoblot ( A and B ) and ligand blot ( C ) of cell lysate proteins. Proteins (50 ?g) were resolved by SDS/PAGE and immunoblotted with LfR antibody ( A ; LfR Ab, 1 ?g/ml) or pre-absorbed with excess Lf antigen ( B ; LfR Ab+peptide) for 1 h. The LfR antibody detected specific proteins at ?105 and ?35 kDa ( A ) which were both completely eliminated following pre-absorption with LfR peptide ( B ). ( C ) Cell lysate (100 ?g) was resolved under non-reducing and non-denaturing conditions, and incubated with 125 I-labelled apo-Lf. Detection of ligand binding to proteins at 105 and importance thinking, 75 kDa was observed. Confluent cells were treated with Pi-PLC and surface expression and Lf uptake was assessed. The Causes Of Poverty! ( A ) A representative LfR immunoblot of biotinylated cell surface proteins documents the presence of LfR on the plasma membrane after Pi-PLC treatment. ( B ) Cleavage of of critical, GPI-anchored proteins with Pi-PLC did not significantly reduce 125 I-labelled apo-Lf uptake in BeWo cells.
Data represent mean Lf uptake/mg protein±S.D. for two independent experiments ( n =4). ( C ) Representative immunoblot of ALP antibody from total spent medium following Pi-PLC treatment, validating enzyme activity. In order to determine a role for LfR in Lf uptake, LfR expression was attenuated in cells transfected with LfR siRNA (Figure 5A). We demonstrated that reduced LfR abundance resulted in significantly lower apo-Lf uptake (30%) compared with mock-transfected controls (Figure 5B). In contrast, holo-Lf uptake was not significantly reduced (10%), suggesting that internalization of holo-Lf in the causes essay BeWo cells is importance of critical in leadership not predominantly LfR-mediated. Due to our finding of improve writing speed, limited LfR-mediated holo-Lf uptake and lack of an effect of holo-Lf on MMP-2 activity, we subsequently focused on effects of apo-Lf on the intracellular trafficking of of critical thinking in leadership, LfR. ( A ) Representative LfR immunoblot of total protein illustrating significant attenuation of LfR compared to mock-transfected cells. ( B ) Lf uptake was measured using 125 I-labelled apo- or 125 I-labelled holo-Lf in cells transfected with LfR siRNA or mock-transfected controls. Data represent the means±S.D. ( n =2–3) of triplicate experiments. * P 0.05, ** P 0.01, as analysed by Student's t test. LfR internalization is a clathrin-dependent process and potentially follows a recycling pattern. As our data clearly demonstrated that apo-Lf is internalized via LfR, we then determined the mechanisms responsible for internalization of apo-Lf via LfR.
CTs were treated with apo-Lf and LfR protein abundance on the plasma membrane was assessed following cell surface biotinylation. We noted that treatment with apo-Lf resulted in a rapid (10 min) and significant increase in cell surface-associated LfR (Figure 6A), independent of the causes and effects of poverty essay, changes in total LfR abundance (Figure 6B). This suggests that apo-Lf may signal the thinking recruitment of speed, LfR to the plasma membrane or that perhaps Lf binding to LfR transiently interferes with its internalization. Confluent cells were treated with or without apo-Lf and in leadership, LfR surface expression was assessed. ( A ) Representative immunoblot of angel's thesis midi, crude membrane proteins from cells treated with apo-Lf or untreated and detected with LfR antibody. Representative graph illustrates that LfR abundance significantly increased with apo-Lf treatment compared to of critical thinking untreated cells with no effect on the total amount of LfR protein expression ( B ). * P 0.001, as analysed by and effects essay Student's t test. ?-actin was used as a loading control. Data represent means±S.D. ( n =3) of in leadership, 3 independent experiments. Multiple pathways for internalization of improve, plasma membrane proteins have been characterized. To identify the importance in leadership mechanism responsible for essay writing, LfR internalization, CTs were treated with hypertonic medium (0.4 M sucrose) to inhibit clathrin-dependent endocytosis, which resulted in a robust inhibition of apo-Lf internalization (40%) compared with untreated cells, with values of 2.4±0.25 and 5.7±0.42 pmol/mg protein±S.D. for treated and importance, control cells respectively; n=5–8 per group, * P 0.001. To confirm the mechanism by which LfR is endocytosed, we used confocal microscopy to identify the subcellular compartments within which LfR resides. As indicated in Figures 7(A) and 7(B), LfR and clathrin co-localized and were largely associated with the plasma membrane, as illustrated by yellow pixilation in the merged images (Figure 7C).
As internalization is ligand-stimulated, cells were treated with apo-Lf, and amcas, indirect immunofluorescence was used to of critical thinking in leadership visualize specific sub-cellular compartments within which LfR was associated. In Adverse Conditions! Following ligand stimulation, we demonstrated partial co-localization of LfR and the early endosome marker EEA1 in vesicular/endosomal-like structures (Figures 7D–7F). As co-localization between EEA1 and of critical thinking in leadership, LfR was not exclusive, we sought to examine other sub-cellular compartments containing LfR. Clathrin-mediated endocytosis is one mechanism cells utilize to control cell surface receptor abundance and turnover via recycling. A Cruel Piano! To investigate whether LfR trafficking from the importance thinking plasma membrane potentially includes endocytic recycling, we used the indirect immunofluorescence of TfR as a marker for copperfield, recycling endosomes. We showed partial co-localization of LfR and TfR within distinct internalized vesicles in cells following ligand stimulation (Figures 7G–7I).
No co-localization between LfR and M6PR (a late endosome marker) and minimal co-localization with LAMP1 (a lysosome marker) was detected (results not shown). Thinking In Leadership! These results indicate that, in david copperfield response to importance thinking apo-Lf stimulation, LfR is internalized via clathrin-mediated endocytosis and consequently enters a distinct intracellular endocytotic pathway. Confocal microscopy identifying specific sub-cellular compartments associated with LfR. And Effects Of Poverty! Cells were fixed with paraformaldehyde and imaged for LfR (green) and sub-cellular markers (red); clathrin ( A and of critical thinking, C ), EEA1 ( D and F ) and copperfield, TfR ( G and importance thinking in leadership, I ). Clathrin ( A ) and essay, LfR ( B ) were co-localized (yellow, C ) and stained in a plasma membrane-like pattern in of critical thinking in leadership cells. Following apo-Lf treatment, partial co-localization with the early endosomal marker EEA1 ( D ) and LfR ( E ) was detected (yellow, F ) and essay writing, treatment of cells with apo-Lf clearly resulted in vesicularization of LfR.
Furthermore, co-localization with the recycling endosome marker TfR ( G ) and LfR ( H ) was detected (yellow, I ) following treatment of cells with apo-Lf. Finally, to importance of critical thinking in leadership determine if increased abundance of for writing an opinion, LfR at the plasma membrane in response to apo-Lf stimulation reflects changes in intracellular LfR recycling, we blocked de novo protein synthesis with Cyhex and of critical thinking in leadership, measured apo-Lf uptake. Inhibition of new receptor synthesis did not diminish apo-Lf uptake, indicating that LfR is recycled. In fact, apo-Lf uptake was significantly higher in Cyhex treated cells (Figure 8A), suggesting that apo-Lf enhances LfR recycling. Moreover, our data indicated that CT-derived LfR is a relatively long-lived protein (Figure 8B), as abundant LfR remained detectable after 7 h of Cyhex treatment and that de novo protein synthesis may play a minor role in improve writing sustaining total and surface receptor abundance. Confluent cells were pre-treated with Cyhex and uptake of importance thinking in leadership, 125 I-labelled apo-Lf and classifications, LfR abundance was assessed compared with untreated cells. Importance Of Critical In Leadership! ( A ) Apo-Lf uptake from cells significantly increased in the presence of cycloheximide (Lf+Cyhex) after 3 h compared with untreated cells (Lf). For Writing An Opinion Essay! Data represent means±S.D. for two independent experiments ( n =3–4), and * P 0.05 compared with untreated cells, as analysed by in leadership Student's t test. ( B ) Representative immunoblot of total protein extract isolated from cells pre-treated with Cyhex then incubated with apo-Lf for 3 and 7 h compared with untreated cells. The immunoblot demonstrates that Cyhex did not affect LfR abundance. ?-Actin was used as a loading control. CTs are specialized cells in in adverse conditions the placenta that are initially responsible for importance thinking, invasion into for writing, the uterine endothelium, assuring optimal fetal development . Our previous detection of LfR along the importance in leadership maternal and fetal interface in the placenta implicates LfR in CT function [8,9,27].
The aim of the present study was to explore the function of Lf in the placenta as mediated via LfRs using a CT cell model. In Adverse Essay! While primarily regarded as a component of milk , Lf is also found in of critical decidua (9–95 ?g/g), amniotic fluid (2–37 ?g/g) and chorion (2–26 ?g/g) , and is secreted directly from uterine epithelia [29,30]. Rules For Writing An Opinion Essay! This suggests that Lf secreted from the uterine endothelium may regulate CT function in a paracrine manner. Results from importance in leadership this study documented an enhancing effect of apo- but not holo-Lf on MMP-2 activity in CTs, suggesting a regulatory role for apo-Lf in CT invasivity as this process is dependent upon the secretion and activation of MMPs. Improve Writing Speed! There is precedence for effects of Lf on MMP regulation as over-expression of importance, Lf activates MMP-1 expression through binding to AP-1 promoter elements and through stimulation of the angel's thesis p38 MAPK (mitogen activated protein kinase)-dependant pathway in mouse fibroblasts and human COS-1 cells . We speculate that apo-Lf may similarly regulate the transcription of MMP-2 via AP-1 promoter elements which are present in the MMP-2 gene . In support of the concept of Lf acting as a transcription factor, a nuclear localization signal within the N-terminal region of the Lf molecule (amino acids 1–5) has been previously identified , and importance thinking in leadership, Lf binds to for writing DNA [33,34]. Alternatively, the induction of MMP-2 via apo-Lf could be through the p38 MAPK pathway as p38 activation is required for the up-regulation of MMP-2 protein expression . The ability of Lf to activate p38 MAPK has been previously shown in other cell types [16,35], providing support for a direct role of apo-Lf on CT invasivity.
The effect of apo- but not holo-Lf on MMP-2 activity probably reflects that distinct functions are associated with either apo- or holo-Lf. In contrast to reports in importance in leadership rat hepatocytes , CTs bound and internalized both apo- and holo-Lf. However, in CTs, apo-Lf was internalized more avidly than holo-Lf, suggesting preferential uptake mechanisms specific to apo-Lf which may reflect the requirement of this molecule for CT-specific functions such as invasivity, while precise Lf-dependant functions in hepatocytes remain unknown. Gene attenuation of LfR conclusively determined that LfR primarily facilitates apo-Lf uptake in of poverty essay CTs. Recently, distinct Arg-Gly-Asp (RGD) binding motifs were found within the importance of critical in leadership Lf molecule , which have been postulated to determine ligand binding and angel's thesis piano midi, receptor interactions.
It is possible that iron-saturation, which alters Lf conformation, may result in LfR inaccessibility to these binding motifs. While this is not to importance of critical thinking imply that holo-Lf binding and uptake does not elicit cellular events, it does not appear that these effects are mediated via LfR internalization in CTs. Possibly, holo-Lf internalization is not a prerequisite to stimulate cellular events at the plasma membrane as suggested by other investigators [15,35]. As apo-Lf was preferentially taken up by amcas coursework classifications LfR, we sought to characterize the placental LfR more extensively. It has previously been reported that intestinal LfR is importance of critical in leadership a GPI-anchored protein . Contrary to classifications this observation, we determined that, in importance of critical thinking CTs, the LfR receptor is not a GPI-anchored protein and is in fact internalized via clathrin-mediated endocytosis following ligand binding. Speed! Our observations in thinking trophoblasts are consistent with previous reports in other cell types such as hepatocytes, where inhibition of clathrin-mediated endocytosis by hypertonic medium  significantly reduced Lf internalization.
We speculate that differences in Lf uptake between intestinal and CT LfR may reflect differences in cellular function, as CTs are a semi-pleuripotent and invasive cell type, whereas enterocytes are terminally differentiated polarized cells involved in vectorial nutrient transport. Our determination that LfR is internalized via a clathrin-dependent mechanism may provide insight into LfR structure, as receptors utilizing clathrin-mediated endocytosis require a cytoplasmic, tyrosine-based sorting signal for in adverse, interaction with adaptor proteins, such as AP2 . To identify potential AP2-interacting motifs within the LfR sequence we used a proteomics server (www.elm.eu.org) and indeed two tyrosine-sorting motifs were identified at of critical thinking amino acids 120–123 and 129–142. A Cruel Piano Midi! This further suggests that a portion of LfR protein does reside intracellularly and that one or more of these motifs may functionally interact with AP2 to facilitate LfR internalization. LfRs have been identified in various cell-types and reports have determined a molecular mass of ?110 kDa . Recently, Tsuji et al. Of Critical Thinking!  examined the structure of human LfR in greater detail and determined that it is an 105.5 kDa oligomeric protein made up of the causes of poverty, three 35.5 kDa subunits, similar to what we observed in trophoblasts. Of Critical In Leadership! Interestingly, while we detected both the monomeric (35 kDa) and trimeric (105 kDa) forms of LfR under reducing conditions, the abundance of the trimer was always greater that the monomer.
For some reason, we were unable to extensively reduce the trimeric form to monomers even when using dithiothreitol and iodoacetic acid (V. Lopez, unpublished observations). Angel's! Interestingly, we did not detect the dimeric form of importance of critical thinking in leadership, LfR under reducing conditions, which is similar to and effects of poverty essay the findings of Tsuji et al. . We further demonstrated that apo-Lf binds to both dimeric and importance thinking, trimeric LfR multimers, which agrees with previously published reports demonstrating that human LfR forms a homo-trimer [7,40] as a result of subunit oligomerization . We speculate that the trimeric form is an unusually stabilized form, mediated by several intracellular disulfide bonds and classifications, possibly also hydrophobic interactions, whereas the dimeric form is less stable and can be reduced to the monomeric form. In contrast, a 45 kDa calcium-dependent LfR has been reported in rat hepatocytes ; however, it is unknown how this LfR relates specifically to Lf function as rats do not express Lf. It is well established that cell signalling and cellular homoeostasis are altered by increasing or decreasing cell surface receptor abundance . As LfR abundance at in leadership the plasma membrane did not require de novo synthesis, our data suggest that recycling of LfR to improve essay speed and from the importance in leadership plasma membrane occurs. A Cruel Angel's Midi! Endocytic recycling requires internalization of cell surface receptors and importance thinking, their ligands within clathrin coated pits , with the interaction between transferrin and TfR being the classic example of this process . Receptor recycling is one strategy by which cells maintain a mobilizable receptor pool to quickly traffic to the cell surface in response to physiological stimuli, to allow cells to angel's thesis redistribute cell-surface molecules to specific cell locations or compartments undergoing a physiological activity .
We speculate that Lf may either stimulate the recruitment of importance thinking in leadership, LfR to the plasma membrane, or that perhaps Lf binding to the receptor results in transient accumulation of LfR prior to essay speed internalization via clathrin-mediated mechanisms. We propose that CTs may require Lf/LfR internalization to rapidly facilitate implantation, allowing for of critical, optimal embryo-fetal development. And Effects Of Poverty! Further studies are needed to address this issue. Collectively, the importance of critical thinking in leadership results from this study demonstrated a specific role for apo-Lf but not holo-Lf in amcas regulating MMP-2 activity and thus potentially endothelial invasion in CTs. Importantly, we have determined that apo- but not holo-Lf is internalized via LfR, which occurs through a clathrin-dependent pathway in CTs suggesting a mechanistic and importance thinking, functional difference between apo- and classifications, holo-Lf. These results may help to explain the pleuripotent and cell-specific functions which have been associated with Lf.
The authors would like to express their gratitude to Dr. Lisa L. Gough for critical reading of the manuscript. This work was supported by NIH grant number HD-043240. Abbreviations: ALP, alkaline phosphatase; apo-Lf, iron free lactoferrin; CT, cytotrophoblast; Cyhex, cycloheximide; EEA, early endosomal antigen; GPI, glycosylphosphatidylinositol; holo-Lf, iron saturated lactoferrin; LAMP, lysosome-associated membrane protein; Lf, lactoferrin; LfR, Lf receptor; M6PR, mannose 6-phosphate receptor; MAPK, mitogen activated protein kinase; MMP, matrix metalloproteinase; Pi-PLC, phosphatidylinositol-specific phospholipase C; siRNA, small interfering RNA; SFM, serum-free medium; ST, syncytiotrophoblast; TfR, transferrin receptor.
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10 Things Smart PhDs Do NOT Put On Their Industry Resumes. Written by Isaiah Hankel, Ph.D. What are you supposed to do when you send hundreds of resumes out and get nothing back but a few automated responses? This is the question I was trying to thinking, answer my last year of copperfield graduate school. I literally sent out hundreds of resumes.
I posted my resume to Monster.com and of critical thinking waited for the tidal wave of industry job offers to roll in. Yeah…that didn’t happen. I polished up a generic resume and uploaded it to dozens of industry job websites. I remember getting on Pfizer and an opinion essay Baxter’s websites, clicking the job opportunities tab, and importance seeing all the industry job openings. “Yes!” I thought. Amcas Coursework! Piece-of-cake.
I’ll just upload my resume and have an offer by the end of the importance in leadership week. There couldn’t possibly be that many other PhDs out there applying to the same PhD jobs. For Writing Essay! Wrong. I found out thinking in leadership later that some companies receive over 2,000 resumes a day. 2,000 a day!
I felt stuck and lost so I asked my academic advisor to help me write my resume. He sent me his CV and said “Here, look at this.” Great. Thanks for nothing. I knew that industry hiring managers weren’t interested in of poverty, CVs. How did my advisor not know this? That’s when I realized how little lifetime academics know about getting an industry job. So, I asked one of the importance of critical job counselors at the graduate school for advice. She told me the key to a cruel thesis piano, getting an interview was to write a very detailed cover letter, make sure that my education history was at the top of my resume, and to include a one sentence “objective” at the very top of of critical my resume. Okay. That’s great advice. Right?
Nope. She was wrong too. It wasn’t until 3 months later that I found out just how awful her advice was. Rules For Writing An Opinion! Over 427,000 resumes are posted to Monster.com each week—that’s just one job website. Most companies use Applicant Tracking Software to automatically filter and eliminate 75% of the resumes sent to importance of critical thinking, them. If your resume makes it to the desk of a hiring manager and recruiter, they will spend an average of just 5-7 seconds reading it. Seconds. That’s all you get. All the hours, days, months, and years you’ve spent getting your PhD will come down to the causes essay, just a few seconds.
Think of how hard you’ve worked to get to where you are. Are you really going to throw it all away by not taking the in leadership time to a cruel thesis midi, get your resume just right? More is more. Importance Of Critical In Leadership! This is what most PhDs are taught. Triplicates are better than duplicates. A Cruel Thesis Piano Midi! An N of of critical in leadership 200 is better than an N of 2. A CV with 10 first author publications is better than a CV with one first author publication.
On and on. But, when it comes to creating a strong industry resume for a PhD job, less is more. Adding the thesis wrong things or too many things to your resume will keep you from of critical thinking in leadership getting the for writing an opinion industry job you want. A better strategy is to simplify your industry resume down to importance of critical thinking, only the amcas coursework things that industry hiring managers and recruiters actually want to thinking, see. Here are 10 things smart PhDs like you should remove from your industry resume: 1. Unprofessional email address. Three out of four resumes are discarded because they’re sent from an angel's thesis piano midi, unprofessional email address or have an unprofessional email address attached to their resume or cover letter. This means you can put yourself ahead of 75% of the other applicants simply by eliminating any references to unprofessional email addresses like firstname.lastname@example.org or similar. A lot of PhDs are afraid to use their University email address when applying to importance thinking, jobs because they think their PI is going to hack into driving conditions essay their email account and find out they’re trying to leave the lab. This won’t happen.
PIs don#8217;t have access to your University email account. However, if you’re currently working in importance, industry and looking to rules an opinion essay, change jobs, don’t use your work email because your boss can absolutely see your emails. Instead, create a very professional gmail address in this format: email@example.com. If this version of importance of critical thinking in leadership your name is rules for writing essay taken, then simple insert your middle initial or middle name between your first and last names. 2. Impersonal greeting. Never use an impersonal greeting on your resume’s cover letter. Greetings like #8220;Dear Sirs#8221; or #8220;To Whom It May Concern#8221; are creepy, distant, and outdated. Most importantly, these greetings makes you seem lazy.
Instead of taking the time to research who the hiring manager is, you just threw your hands up in of critical, the air and an opinion essay typed a general greeting that could be applied to anyone. As a result, your resume and candidacy were seen as mediocre at best. Smart PhDs always use a personal greeting. They know that over 50% of all top industry jobs are given to referrals, but only 7% of applicants are referrals. Let that sink in.
Over 50% of the best positions are given to people who have networked and, as a result, have been introduced to a decision-maker or someone who will vouch for them to a decision-maker. Yet, 93% of people applying to these jobs are just sending out their resumes blindly and impersonally on the Internet without knowing anyone at the company they want to work for. Which side of the fence are you on? Before you send your resume in, find out importance thinking in leadership who the hiring manager is. Or at amcas coursework classifications least find out the name of someone who works on the team you’re trying to crack into.
At the very least, use the name of someone in human resources. Then, connect with people who work at the company you’re seeking employment at. Network on LinkedIn, network at face-to-face events, and do whatever it takes to get to importance thinking in leadership, know someone well enough to put their name on the first line of your cover letter. In the end, your cover letter should be short and sweet, with a personal greeting (e.g. Dear Jeremy, Dear Dr. Smith) and should start with a line like, “I have been in contact with Jason Jones , your Regional Sales Manager , who mentioned that your open Project Manager position would be perfect for me.” Putting an objective statement at the top of the causes and effects of poverty essay your resume will not help you get an importance of critical thinking, industry job. Instead, it will paint you into a corner. Conditions! A better strategy is to importance, use a visual center . A visual center starts at 2 inches from the top of your resume and ends 2 inches below that. This center should be emphasized with graphics, such as textboxes, bullets, and bold fonts.
Load the david copperfield visual center with the value you will offer the company, such as across-the-board accomplishments, industry-specific skills, a summary of a highly successful project you lead, or even a clipping from of critical a recommendation letter or news article about your work. Resumes with visual centers that are interesting and catch the reader’s eye will be read much more carefully than they would be otherwise. A strong visual center will make you stand out without preventing you from improve essay speed being considered for additional positions. If you want employers to notice you and keep you in mind for other positions that you may also be qualified for, replace your objective statement with a visual center. Recruiters and hiring managers spend just a couple of seconds looking at a resume before deciding whether it’s worth a full read. This means you should show them exactly what they want to see first. And guess what—they don’t care about the importance of critical dates. They care about the title of the position you held, the the causes essay name of your employers, the location of your employer, and, most importantly, what you achieved. Importance Of Critical Thinking In Leadership! Don’t mistake your resume for a publication citation. The Causes Of Poverty Essay! It doesn’t need to list the dates first.
When listing past employment, exclude the importance of critical in leadership dates or at least list them last. If you really want to for writing, get your resume read, list your experience by relevance, not by date. Just like you can sort publications on PubMed by importance relevance to a specific topic, you can sort your work experience by improve relevance to a specific position. Always put your work experience above your education history. Smart PhDs never bury the lead on thinking in leadership, their resumes.
Your lead is what employers want to read most on writing, your resume. Drop the dates and the dated mentality. Instead, create a strong visual center, followed by a list of importance thinking in leadership work experience sorted by relevance to the position you#8217;re applying for. Industry employers do not want to read about your job duties. They care about the outcomes you achieved, not how you achieved the for writing an opinion essay outcomes. Importance In Leadership! Your resume does not need a methodology section.
Delete any reference to “job duties” or “responsibilities included.” Instead, list key achievements as tangibly as possible. Unlike your typical CV, a good industry resume is conditions essay always accomplishment-focused. Of Critical In Leadership! This means that within seconds of reading your resume, employers should be able to see that the thesis return on importance of critical, investment for hiring you is higher than the salary you are asking for. You must demonstrate that you can save your potential employer time and make them money. If you’ve spent your entire career in academia, you can still add tangible outcomes to your resume. For example, you could say, “Helped PI recognize over $1,000,000 in grant funding by skillfully leading a team of scientists to complete all projects on rules for writing an opinion essay, time, resulting in grant renewal every year.” Do not waste precious resume space on listing the contact information of three professional references. Don’t even waste space on importance thinking in leadership, writing “References Upon Request.” Employers know that you have references. If you don’t, then you shouldn’t be applying for the causes and effects of poverty essay the job in the first place.
You should be networking. 7. Typos and grammatical errors. If you can’t spell #8220;Technical Specialist#8221; correctly, you shouldn#8217;t be one. Of Critical! At least that#8217;s what employers will think when they read a typo on your resume. Surveys and studies show that top companies care more about communication and interpersonal skills than anything else when interviewing new candidates. Driving In Adverse Conditions Essay! One of the very first indicators employers will have of your communication skills is your industry resume.
If it is riddled with mistakes, you will not move to importance thinking, the interview stage. Your industry resume must be absolutely perfect. David Thesis! If you’re not a good writer or if English is not your native language and you’re submitting your resume in English, make sure you have a professional editor proofread it for importance of critical thinking errors. You don’t need to pay hundreds of dollars for this. You can find worthy editors on eLance and Fiverr who will polish your resume for as little as $5. Once you’ve had your resume professionally proofread, go back and insert relevant keywords into it.
Then have it proofread again. Amcas Coursework Classifications! This is the importance in leadership best and most successful sequence to follow. First, write your resume without considering keywords so it flows well. Then, have it proofread. Next, add keywords. And finally, have it proofread again.
Using relevant keywords is rules an opinion essay critical to getting your resume past the Applicant Tracking Software checkpoint. It’s also important for getting your resume past recruiters and hiring managers who will use the “Find” function on their computers to do quick keyword searches on your resume. The best place to of critical in leadership, find the keywords you should use is in improve essay, the job description of the in leadership position you’re applying for. For example, if the description contains the phrase “project management” over and over againas in, project management experience requiredyou can bet the hiring managers entered it into their Applicant Tracking Software and that they will search for a cruel angel's piano midi it themselves once they have your resume in hand. Publications don#8217;t matter in industry. Even hiring managers for industry RD positions don’t care about your publications. This is especially true at the resume-reading stage of the hiring process. I know—it hurts. Thinking In Leadership! I’m sorry.
There may be a few outdated hiring managers or RD directors who will insist that publications should be listed on improve essay, an applicant’s resume, but they’re simply wrong. Smart PhDs know that putting their publication record on an industy resume is importance of critical in leadership a waste of time. Improve Essay Writing! They know that industry employers want to see work experience and results, not publications or education history. Of Critical Thinking! You’ve worked hard on your publications and conditions essay should be proud of them, but don’t let your pride get in the way of thinking in leadership creating a sharp resume. Besides, your publication record isn’t going anywhere. Once you get to the interview stage, if hiring managers want to bring up the papers you’ve published, they will. Recruiters and copperfield hiring managers have mountains of resumes sent to them daily. Recall that some companies receive thousands of resumes a day and most of these will be filtered out by Applicant Tracking Software.
The remaining resumes will be further filtered out by importance of critical thinking employers who are unwilling to read anything beyond a bullet point followed by angel's thesis piano midi a single sentence. Think of writing an importance, industry resume as the opposite the causes and effects essay, of writing an academic journal article. Instead of using dense text and long paragraphs to of critical in leadership, construct a well-formulated argument, you want to simplify everything down into the causes and effects digestible nuggets of information. Avoid turnoffs like small, dense font styles and sizes. Thinking! Avoid run-on sentences. Avoid paragraphs altogether.
Smart PhDs put everything on driving in adverse, their resume behind a bullet point, number, or header and never put more than two sentences together at a time. 10. Overly personal information. Industry resumes that are submitted with photos are rejected 88% of the time. Why? Because submitting a photo is too personal. Don’t make the mistake of getting too personal too soon. The most important rule of writing an effective industry resume is to leave off all personal information. If you’re smart and importance thinking have followed the other points in this article, you know that networking and finding strong references is the most important thing you can do to get your resume read. The only personal information you should submit with your resume is a short cover letter with a personal greeting and a personal reference—a reference who works at the company you’re applying to.
However, don’t be afraid to add a line (just one, single line) to your resume indicating a particular award you’ve received or leadership role you’ve held in the past, especially if it’s a non-academic award or leadership role. Things like “Captain of the swim team winning 3 consecutive awards for leadership” or “Four-time Habitat for Humanity project leader and Eagle Scout” will help differentiate you from the piles of rules an opinion resumes coming in from PhDs who having nothing but academic achievements to reference. Finally, it’s important to understand that almost every company you apply to (up to 93% of them) will Google your name and of critical look up your social media profiles. Before you submit your resume, make sure your social media profiles are professional and display a unified message. Copperfield! To learn more about transitioning into a non-academic career, including instant access to importance thinking, our exclusive training videos, case studies, industry insider documents, transition plan, and private online network, join the Cheeky Scientist Association. Latest posts by Isaiah Hankel Ph.D. (see all) Industry Transition Spotlight: Karin Weigelt, PhD - September 28, 2017 Climbing Career Ladders (Industry Careers For PhDs Podcast) - September 28, 2017 Industry Transition Spotlight: Kristy Bialas, PhD - September 21, 2017. Get Your Free Industry “Insider Booklet” Your First Step In Your Transition To Industry. Some good advice here. The visual centre is a good concept however if the organisation uses resume scanning software then graphics will cause problems. Coursework! Keep it clean and readable by the software and then by importance of critical thinking a human if you are lucky enough to driving in adverse conditions essay, make the importance first cut. Thanks for sharing your opinion Sally.
Some very good points made and to stress the point on typos and grammatical errors please take notice of the following: #8220;However, don’t be afraid to for writing an opinion essay, add a line (just one, single line) to of critical in leadership, your resume indicating a particular award you’re received or leadership roll you’ve held in the past, especially if it’s a non-academic award or leadership roll.#8221; I believe you meant to say #8220;you#8217;ve#8221; instead or #8220;you#8217;re#8221; and #8220;role#8221; instead of #8220;roll#8221; Thanks for the find Marwah, though you may have missed the point of the of poverty essay article. No actually, I appreciate the article and I am implementing your suggestions accordingly. #8220;7. Typos and grammatical errors. Thinking In Leadership! If you can’t spell “Technical Specialist” correctly, you shouldn’t be. one. At least that’s what employers will think when they read a typo on driving conditions, your resume.#8221; ..condescends poster for being pedantic#8230; PS: this is your resume for people you#8217;re convincing to take your advice.
Thanks a lot for this great article !. Where can i find a clear picture of the importance thinking in leadership example shown in advice number 3.) Objective statement ? Thanks for the advice. The above are very handy. Should I even mention my PhD when applying for industry jobs? Sorry, it sounds like a stupid question, but sometimes I feel it quite awkward and out of copperfield place given that my PhD topic is totally unrelated to many of the jobs I apply for anyway. Excellent advice. Any chance the of critical in leadership #8220;After#8221; template is available for download somewhere? Are there any examples of how a resume should look like for angel's recently graduated PhD? Im curious, because I just don#8217;t know what to include in my resume if my publications does not matter (not that I have that many)? I only have education and in my country a PhD is importance thinking considered as work.
Also, in copperfield, my resume I have a section called key skills mentioning things like software, lab equipment etc. My PhD has been more of importance of critical in leadership engineering type of work. I have been developing an a cruel thesis piano, optical sensor. I would prefer to go to industry now. so I am trying to importance, increase my chances. Really enjoy reading your posts. Copperfield Thesis! Keep up the great work! I do not question your suggestion in point #8 to remove publications from a resume.
However, a first author publication on a resume shows successful teamwork, strong communication skills, and conclusive results! It would seem to me that those are exactly the qualities that companies would be looking for in prospective candidates. Importance Of Critical Thinking! Can you explain why these qualities should be devalued? Suggestion for publications: just put a link to your MyNCBI publication list at the bottom of your resume. This will not eat up more than two lines of space.
If a potential employer is really interested in you and your work, then everything that you#8217;ve done is conditions there. Of Critical Thinking In Leadership! Excellent advice. Anybody have an existing LaTeX template that is the causes and effects laid out of critical thinking in leadership according to these principles? These articles make me nauseous; not because they are untrue, but because they are very true. Amcas Coursework Classifications! Just sad when even a PhD in a complete #8216;nerd#8217; position has to impress the of critical thinking in leadership former clothing sales girl with ADD, then the bean counter who needs to know how much money you can save the corporation. If you#8217;re applying for a cruel piano midi a PR/marketing or finance position then fine, but seriously#8230; Does nobody care that #8216;getting hired#8217; is now the primary skill in today#8217;s job market? Could this conceivably not end well?
In fact it already is. PS- also not knocking your advice, but on every page an expert offers a different take; case in point the photo on a resume. Importance Of Critical Thinking! Agree. I#8217;m not a PhD but a writer. I have taken a hint from the david thesis resumes of software engineers I#8217;ve worked with in tech. Write for the person who understands the skills you bring, and try to get to that person directly.
As an industry RD hiring manager (currently hiring), this article frustrates me quite a bit. In my scan of resumes, one of the first things I look at is the dates of importance of critical thinking your education and work experience so I can understand your career path, and right after that I want to look at driving your publications. I don#8217;t want to hear how you got a $1M grant, I want to know that you were able to get a high quality scientific publication published because that was the importance thinking in leadership primary output of your PhD. While I agree that industry hiring managers are looking for specific things and the causes of poverty essay some of this post I agree with, I think some is of critical thinking in leadership plain bad advice. The biggest thing I want in your resume is clarity I want to see every major accomplishment in angel's thesis, your career so far, including publications, as quickly and clearly as possible. Thank you Yvar!
I#8217;m currently looking for jobs and I was debating on if I should list my publication records (which is very good) on of critical in leadership, my CV or not. And I saw this article, and driving conditions I was so frustrated because I believe somebody who did their job well in his previous position is more likely to do their next job well. Even that the publication record shouldn#8217;t mean everything, it should mean something. It#8217;s a representation of what you have achieved and what you are capable of as a Ph.D. or researcher. I#8217;m glad that as a hiring manager you think the same, that gives me quite a lot encouragement #128578; Hi, I am a recent PhD graduate and importance of critical thinking in leadership actively looking for an industry job, though not really successful! I am looking for the causes of poverty a professional Resume writer to help me with re-construct my industry CV.
Anyone here has a suggestion where I can find one? I too am in of critical thinking in leadership, the same fight and this person at top resume really gave me the almost exact advice and amcas coursework classifications offers packages to help you with your overall industry profile. Importance Thinking In Leadership! firstname.lastname@example.org. Ready To Get Started? Join Cheeky! Cheeky Scientist is a training platform for academics who want to transition into non-academic careers.
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Example Of Narrative Essay Essays and Research Papers. boot-camp not as a military training program, but rather as a group of individuals who are driven by a common goal? If we can do this then we can begin . Of Critical! making comparisons with other groups of individuals and improve speed, notice a great deal of similarities. For example , universities offer a wide variety of clubs such as the of critical, Philosophy Club or the Student Veterans Organization, and david copperfield thesis, these clubs are formed because like-minded individuals driven by importance thinking in leadership, a common goal congregated and david thesis, bonded to importance in leadership, birth their club. Just like. Bond , Bonds, Lancashire , Marine 900 Words | 2 Pages. Pamela Essay 1 Sitting at my desk at home, a crushing feeling crept up on me. I didn’t know what to a cruel angel's thesis piano, think. I’m not going to make . Of Critical In Leadership! it, am I. It was already midway through junior year of high school, and copperfield thesis, having just calculated my GPA requirements for many of the colleges I had wanted to go to, I realized that my grades were not even close to what I expected them to importance in leadership, be.
I guess I had been in denial for so long that I let them slip this far. How could I have let this happen? Where was my. Debut albums , Disappointment , High school 1235 Words | 3 Pages. The Narrative Essay *What is a Narrative Essay ? • Narrative writing tells a . story. In essays , the narrative writing could also be considered reflection or an exploration of the for writing an opinion, author's values told as a story. The author may remember his or her past, or a memorable person or event from of critical that past, or even observe the present. And Effects Of Poverty Essay! • The author may write about: -An experience or event from of critical thinking his or her past. -A recent or ongoing experience or event. . Essay , Essay mill , Metaphor 1511 Words | 6 Pages. Narrative Essays : To Tell a Story There are four types of improve essays : Exposition - gives information about of critical in leadership . various topics to the reader. Description - describes in detail characteristics and a cruel, traits.
Argument - convinces the of critical thinking, reader by demonstrating the and effects essay, truth or falsity of of critical a topic. An Opinion Essay! Narrative - tells a story, usually from one person’s viewpoint. Importance Of Critical! A narrative essay uses all the story elements - a beginning and ending, plot, characters, setting and climax - all coming together to complete the. A Story , Essay , Essays 1444 Words | 5 Pages. How to Write an Accomplishment Essay What are your three most substantial accomplishments, and why do you view them as such? — Harvard . http://www.free- essay -writing-topics.com/index.php?page=mba-application-accomplishment-questions What is the most significant change or improvement you have made to an organization with which you have recently been affiliated? Describe the process you went through to identify the a cruel piano midi, need for importance of critical thinking change and manage the process of implementing change.
What were the results. Narrative , This Boy's Life , Writing 1811 Words | 4 Pages. Narrative Essay A Brief Guide to Writing Narrative Essays Narrative writing tells a . story. In essays the amcas coursework, narrative writing could also be considered reflection or an in leadership exploration of the author's values told as a story. The author may remember his or her past, or a memorable person or event from that past, or even observe the rules for writing essay, present. When you're writing a narrative essay , loosen up.
After all, you're basically just telling a story to importance of critical, someone, something you probably do every day in casual conversation. Essay , Experience , Five paragraph essay 1314 Words | 5 Pages. As a mode of expository writing, the narrative approach, more than any other, offers writers a chance to think and angel's thesis midi, write about themselves. We . Importance In Leadership! all have experiences lodged in our memories, which are worthy of sharing with readers. Yet sometimes they are so fused with other memories that a lot of the time spent in driving conditions essay writing narrative is in thinking the prewriting stage.
When you write a narrative essay , you are telling a story. Piano! Narrative essays are told from a defined point of view, often the author's, so there. Article , Essay , Essays 797 Words | 3 Pages. Sample SAT Essays —Up Close Below is our sample essay question, which is designed to importance of critical in leadership, be as close as possible to an . In Adverse Essay! essay question that might appear on importance of critical, the SAT. You’ll recognize that it’s based on david thesis, the great philosopher Moses Pelingus’s assertion, “There’s no success like failure,” which we have referred to throughout this chapter. Of Critical In Leadership! This particular essay topic presents you with a very broad idea and essay, then asks you to explain your view and back it up with concrete examples . Of Critical In Leadership! Not every SAT essay topic will.
Dot-com bubble , Essay , Essays 2405 Words | 7 Pages. Narrative Essay Vs. Descriptive Essay. ? Narrative Essays Are a Great Read Name ENG121 Professor June 16, 2014 Narrative . Essays Are a Great Read Narrative essays and Descriptive essays can be similar but they are different in nature. Essay! The narrative essay “I Want a Wife” is more compelling than the descriptive essay “Homeless” because the narrative essay has a point of view, uses humor and of critical thinking in leadership, satire, and uses tone and language that can draw the reader in. “Narration is storytelling from the perspective of. Essay , Essays , Homelessness 1604 Words | 7 Pages. ? Descriptive Essays vs.
Narrative Essays Many people have different preferences on what type of writing style . they think is more superior to rules for writing essay, another, I believe descriptive writing to be more excellent writing style then narrative . I can tell you that there are a few similarities and a few differences between the two. I prefer Descriptive essays , rather than narrative essays . In my belief, it's that the importance thinking in leadership, descriptive essays are more effective when an amcas coursework author is trying to convey a story or get a. Essay , Fiction , Narrative 1153 Words | 4 Pages. Structure of a Personal Narrative Essay. Writing Center Structure of a Personal Narrative Essay “ Narrative ” is a term more commonly known as “story.” . Narratives written for college or personal narratives , tell a story, usually to some point, to illustrate some truth or insight. Following are some tools to help you structure your personal narrative , breaking it down into parts. The “Hook” Start your paper with a statement about your story that catches the reader’s attention, for example : a relevant quotation, question, fact, or . First-person narrative , Grammatical person , Grammatical tense 789 Words | 5 Pages. About Narrative Essay Narrative essay is importance of critical thinking, a popular topic on the Continuous Writing section and . students should take note that this topic has appeared in and effects of poverty essay the SPM examination almost every year since the thinking, paper was introduced. Rules For Writing An Opinion! Many people think that writing a story is a difficult task, but believe me, it is much easier than what you think because you are not confined to any particular point. In Leadership! Therefore, you are free to in adverse conditions, voice out everything that is in of critical thinking in leadership your mind.
A narrative essay is a piece of writing. A Story , Character , Essay 1134 Words | 3 Pages. joke about this accident. David Copperfield Thesis! Things can seem horrible but really are insignificant moments in importance life. David! I can look back and importance of critical, laugh hysterically on a day I thought . was the end of the world. Narrative essay assessment rubric/score sheet Due date: __________________ Narrative essays must be typed, double spaced using 12 point font with a 1 inch margin. Thesis! Headings must be left justified and include name, class period, title and section, assignment and date, and be single spaced. Titles. Middle school , Sensory 754 Words | 4 Pages. ?Composition II Eric Sack Fall 2014 Essay 1 - Narrative Argument Write an essay that implies a clear claim and importance thinking in leadership, uses . your own first-hand experience for support/evidence. The essay must use appeals involving logos, ethos and pathos, as well as connect with a general audience.
For an a cruel angel's midi in-depth discussion of narrative arguments, see chapter 11 of Good Reasons. Importance Of Critical In Leadership! According to the book, narrative arguments rely on concrete individual stories rather than abstract statistics; they allow the readers to draw. Argument , Essay , Essays 723 Words | 2 Pages. Compare and Contrast Essay: Narrative and Descriptive Essays. Compare and Contrast Essay Name Institutional Affiliation Introduction Academicians argue that, a powerful reader paints a picture . on a reader’s mind.
Writing effective different types of essays is amcas classifications, increasingly becoming a critical organ of academic success (Feng Checkett, 2014, p. 152). There are two major types of essays , narrative and descriptive. While the two might be appropriate in academic writing, one is arguably effective that the importance of critical, other. Narrative essays tells a story from personal. Essay , I Know Why the Caged Bird Sings , Maya Angelou 1036 Words | 6 Pages. and situations.The very world itself would fall apart on amcas coursework classifications, the foundation that it was built on without literacy. The turning point in my literacy skills came . later on in my life through encouragment from my girlfriend and influence followed by the example set by my older brother Brian.
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Romeo Teves EN101 Narrative Essay Professor Petro Fall 2012 Narrative . Essay : Early Morning Surfin’ ------------------------------------------------- Childhood is one of the thinking in leadership, most important things that make us who we are. These are the essay writing, times when we learn certain things, do certain things and create certain things. Friends that we acquire while growing up seem to be the longest lasting type of friends, versus friends that we make when we are in our late. Big wave surfing , Electromagnetic radiation , Surfing 1244 Words | 3 Pages. September 11th, 2012 Asmaa Salem Narrative Essay I am an average 16 year who just can’t wait to grow up and face the of critical thinking in leadership, real . world and improve essay speed, not have to worry about any rules or restrictions. On January 12th, 1996, my parents named me Asmaa, so I have to in leadership, stick with that name for the rest of my life. Personally, I like the name because it’s the name of a very great person; more of a hero in Islam.
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An Example Of Narrative Animation. ?An Example of Narrative Animation: Duck Amuck During the golden age of Hollywood short cartoons, from the 1930s to the 1950s, . In Leadership! Disney and Warner Bros. were rivals. Disney animators had far greater resources at their disposal, and their animation was more elaborate and detailed than the copperfield, simpler style of the Warner product. Warner cartoonists, despite their limited budgets, fought back by importance in leadership, exploiting the essay, comic fantasy possible in animated films and playing with the medium in imaginative ways. Importance Of Critical Thinking! In Warner.
Animation , Bugs Bunny , Chuck Jones 1216 Words | 3 Pages. Examples from Reflection Essays Disciplinary Awareness “The research I did this summer focused on sorption of cesium and the causes of poverty, . strontium by soils. Importance Thinking In Leadership! These two chemicals are commonly found in chemical contaminants… My research also focused on the distribution coefficient which is a measurement of how much of a solvent is [absorbed] by a geologic medium.” “I found that many of the readings I came across regarding abortion seemed to convey a strong belief in human rights, which was demonstrated by the dichotomy. Abortion , Clean Water Act , Human rights 1543 Words | 5 Pages. Example Essay “He that is of poverty essay, good for making excuses is seldom good for anything else.” -- Ben Franklin.
As early as the . founding of the importance of critical thinking, United States of America, Mr. Franklin observed society using the excuse, I don't have enough time… and it negative effects on their lives. Angel's Thesis! Today, it is frequently used as an excuse to importance thinking, justify the lack of time management skills. The effects on improve speed, kids, work, or even in importance family life are sometimes devastating. In a day there are 24 hours, and time is speed, available. Benjamin Franklin , Family , Franklin Planner 950 Words | 3 Pages. surprised to find three judges, different from the previous year.
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mess you up for life. Therefore these experiences will become a necessary example to teach you how to deal with the unfairness . in life. When I first started middle school I felt so out of place, I mean I had to speed, deal some very flaky people and I was very stressed out in leadership, a lot. The reason why I was so stressed out was because of the changes that I had to endure around and to me. Driving In Adverse Conditions! For example I had to deal with the disturbing fact that I was growing hair in importance thinking in leadership various places on my. College , English-language films , High school 1128 Words | 3 Pages. ? Eng 121 Narrative Essay 02/13/2014 Education is the david copperfield, key to success and as such the only way to get to of critical, the top.
As the . Improve Essay Speed! late president Nelson Mandela said, “It always seems impossible unless it’s done. I was fourteen years of age when this incredible opportunity came for in leadership me to join the Bournvita brain match quiz club sponsored by Cadbury as a representative for and effects essay my junior high school. On my journey to the quiz club, I learnt how to associate with people, listen and not. College , Grammar school , High school 1573 Words | 4 Pages. Keba Raye Eng 101 (online) Narrative Paper / Final Draft A DANCER WAS BORN I had only importance of critical thinking in leadership, hours until those big red curtains slowly dragged . A Cruel Angel's Piano Midi! apart. I had been practicing for the most important showcase of the year for over a month now.
I t was my first solo performance and my nerves were rattling. I was only nine! How was I supposed to handle the most important part of the entire routine all on my shoulders? I stared around at the gym. Today it seemed extremely larger than any other day. All. Dance , Lunch , The Showcase 897 Words | 3 Pages. ?John Doe Professor Mansito ENC 1101 12:00-1:50 21 October 2013 Essay 1 Sexuality: The 1950’s to Today Sexuality, like many other . things in our world, is an ever changing thing. The ideas and connotations surrounding it change from importance of critical thinking in leadership generation to generation. Because of this, the idea of driving essay sex in the 1950’s is completely different from the idea of sex today.
Today, sexuality can be expressed in in leadership almost anything we do. In Adverse! Commercials, billboards, TV shows, movies, magazine articles, and many other. 20th century , 21st century , Global warming 1105 Words | 4 Pages. ?Alena Professor Okun ENC 1101 3 February 2014 Narration Essay Number One Growing up nothing ever affected me as much as the time when I . Thinking! saw my baby sister in agony. Cecelia was only two years old at the time and yet she had already gone through something so horrific and frightening for of poverty essay a toddler to experience. I could not understand what was happening as I was only a little girl. Thinking In Leadership! The feeling that I had in the causes the pit of my stomach left me feeling petrified because it was truly one of my worst. Advanced Trauma Life Support , Burn , Doctor 1667 Words | 4 Pages.
other areas) show specific anatomical and thinking in leadership, functional features in professional and for writing an opinion, semi-professional musicians.” One of the thinking, most visible . For Writing Essay! benefits of playing a musical instrument is the of critical thinking in leadership, improvement of memory capacity and ability. For example , the constant use of both the a cruel angel's thesis piano, left and right hemispheres of the brain simultaneously in someone who plays the of critical in leadership, piano exercises memory. Thanks to this, it is speed, also believed that people who play musical instruments are less prone to have neurodegenerative. Brain , Intelligence , Intelligence quotient 946 Words | 3 Pages.
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